agrobacterium mediated transformation of maize (zea mays l.)

نویسندگان

h. rahnama

چکیده

agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. this gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. in the present work, we studied maize transformation using a. tumefaciens by identifying some important factors that affect transformation efficiency subsequent tissue culture and regeneration of transgenic plants. although, all genotypes produced embryogenic calli, s61 and a188 lines had higher percent (75% and 71% respectively) of regeneration than the other ones. transformant events obtained when immature embryos (1.5-2 mm) were inoculated with a. tumefaciens lba4404 harboring a standard binary vector pcambia3301 after 72 h pretreatment culture of the embryos. polymerase chain reaction (pcr) confirmed the presence of the gus and bar genes in the genome of regenerated plants. the transformation frequency (the number of independent, pcr-positive transgenic plants per 100 embryos infected) was 6.45% for s61 genotype. therefore, our results identified suitable genotype (s61), embryo size (1.5-2 mm), a. tumefaciens strain (lba4404), pretreatment culture, and appropriate antibiotic (timentin) for agrobacterium mediated transformation of z. mays.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Agrobacterium Mediated Transformation of Maize (Zea mays L.)

Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...

متن کامل

Agrobacterium Mediated Transformation of Maize ( Zea mays L . )

Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...

متن کامل

Agrobacterium-mediated transformation of maize (Zea mays) immature embryos.

Agrobacterium tumefaciens-mediated transformation is one of the most efficient and simple gene delivery systems for genetic improvement and biology studies in maize. This system has become more widely used by both public and private laboratories. However, transformation efficiencies vary greatly from laboratory to laboratory for the same genotype. Here, we illustrate our advanced Agrobacterium-...

متن کامل

Maize (Zea mays L.).

Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...

متن کامل

Maize (Zea mays) Hi-II Transformation via Agrobacterium-Mediated T-DNA Transfer

Genetic transformation of maize via Agrobacterium tumefaciens is still more art than science, with different researchers achieving substantially different transformation results. This article describes our advanced Agrobacterium-mediated transformation system in Hi-II maize. The system utilizes simple binary vectors and immature embryos for the transformation, employing the bar gene as a plant ...

متن کامل

Agrobacterium tumefaciens-mediated transformation of corn (Zea mays L.) multiple shoots

An Agrobacterium tumefaciens-mediated corn transformation method based on multiple shoot tissue cultures was developed, which is effective with a variety of corn inbred lines and standard binary vectors. Six factors that affected the success of corn transformation were tested, including A. tumefaciens strain, corn genotype, tissue culture growth stage, medium composition, co-culture temperature...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of sciences, islamic republic of iran

ناشر: university of tehran

ISSN 1016-1104

دوره 21

شماره 1 2010

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023